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KMID : 1161520130170020106
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Animal Cells and Systems
2013 Volume.17 No. 2 p.106 ~ p.112
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Potential use of adult bovine serum obtained during the slaughtering process as a biological reagent
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Yu Ji-Eun
Kim Min-Soo
Pokharel Smritee
Kim Ji-Hoe
Choi In-Ho
Choe Nong-Hoon
Nahm Sang-Soep
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Abstract
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Starting from 2013, the Korean ‘Marine Environment Management Act,’ in response to international regulation on marine pollution, will prohibit the marine disposal of land wastes such as animal husbandry by-products and waste produced during the slaughtering process. Currently, most bovine blood is wasted during the slaughtering process and causes environmental problems in Korea. Therefore, long-term measures are required to process blood waste produced during the slaughtering process. The aim of this research is to investigate the potential use of adult bovine serum obtained during the slaughtering process for use in cell cultures. Primary mouse astrocytes, primary human fibroblasts, and L6 cells were cultured with adult male, adult female, and castrated male bovine sera for comparison with fetal bovine serum (FBS). The performance of adult bovine serum was comparable to that of FBS in culture of astrocytes and fibroblasts. Compared to FBS, adult bovine serum supported enhanced proliferation and differentiation of L6 cells. These results suggest that adult bovine serum obtained from the slaughtering process has a potential use as a biological reagent. Moreover, recycling adult bovine serum will contribute to solving the environmental issues arising from the disposal of waste produced during the slaughtering process.
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KEYWORD
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bovine serum, blood chemistry, cell culture
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